Gene expression analysis techniques for stem cell characterization
Human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC) can differentiate into various cell lineages including bone, cartilage, fat, muscle, and neurons.
SLAS 2024: Robots, robots, and more robots!
The main event of the Society for Laboratory Automation and Screening (SLAS) is its annual International Conference and Exhibition which rotates between Boston and San Diego.
On HTS: Hit Selection
There are two strategies to select hits. You can either rank the samples based on their effect size in the assay and pick top performers or you can pick samples that meet the pre-set threshold. In either case, the goal is to maximize true-positive rates…
On HTS: Z-factor
Imagine you do a high throughput screening campaign, how do you know your assay is reliable enough to generate meaningful hits? Well, one might use signal-to-background (S/B) or signal-to-noise (S/N). However, both parameters fail to fully capture the variability in the sample and the background. Zhang et al. introduced the Z-factor in 1999 to address …