On HTS Hit Selection

On HTS: Hit Selection

There are two strategies to select hits. You can either rank the samples based on their effect size in the assay and pick top performers or you can pick samples that meet the pre-set threshold. In either case, the goal is to maximize true-positive rates…


On HTS: Z-factor

Imagine you do a high throughput screening campaign, how do you know your assay is reliable enough to generate meaningful hits? Well, one might use signal-to-background (S/B) or signal-to-noise (S/N). However, both parameters fail to fully capture the variability in the sample and the background. Zhang et al. introduced the Z-factor in 1999 to address …

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On High-throughput screening

High-Throughput Screening (HTS) stands as a game-changing technique revolutionizing drug discovery and scientific research. With its rapid and systematic approach, HTS enables researchers to assess a vast number of compounds for their biological activity in a short span of time. This accelerates the identification of potential drug candidates and facilitates the exploration of various target …

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