Gene expression analysis techniques for stem cell characterization
Human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC) can differentiate into various cell lineages including bone, cartilage, fat, muscle, and neurons.
Assay
Assay of the Week: Disabled Insecticidal Protein (DIP) assay
Assay of the Week: Disabled Insecticidal Protein (DIP) assay Application: Screening for the alternative MoA (different insect receptors binding) of insecticidal pore-forming toxins. Field: AgBiotech, entomology, protein science. Background: Cry proteins, also known as crystal proteins, are a class of insecticidal proteins produced by the bacterium Bacillus thuringiensis (Bt). These proteins/toxins are notable for their …
Assay of the Week: Disabled Insecticidal Protein (DIP) assay Read More »
On ELISA: Calibration Curve
King of Immunoassays, ELISA! Probably, there is no need to explain the importance of ELISA here! I am going to have a few posts on ELISA to capture my thoughts and experience. Hopefully, they help others. The ELISA data quality is as good as the quality of the standard curve. So, let’s start this thread …